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AmpliStain OPEN™ - novel detection systems for ImmunoHistoChemistry (IHC) and Colorimetric In-Situ Hybridization (CISH) - from the original developer and sole manufacturer of the PolyHRP Conjugates Launched in 1990, PolyHRP predated dextran and other backbone polymeric conjugates. However, PolyHRP was developed primarily for UltraSensitive ELISA and is not applicable in IHC staining protocols as it can not permeate into tissue sections because of its larger size. With PolyHRP as such SDT has regretfully been longer time inactive in addressing IHC marketplace needs. While belatedly, now we offer a comprehensive line of the enhanced detection products for the most demanding IHC applications. We are sure that a long way made by AmpliStain™ and StrongZyme™ in reaching IHC customers will be paid off by the excellent high quality performance of our products along with their well recognized consistency, stability and robustness. We offer products for 1-Step immunostaining (read more) OPEN™ stands not only for Oligo-Polymer Enhancement, but also has a direct meaning. Taking into consideration diversity of routine clinical diagnostic and experimental research IHC applications along with very different customer work traditions we supply all AmpliStain™ reagents with no fixed use instructions. AmpliStain™ is an open system suggesting unrestricted variety of tailored custom protocols optimized by ImmunoHistoChemists addressing their specific needs. All AmpliStain™ reagents supplied in Ready to Use RESERVE™ format, i.e. at maximum working strength for High Sensitivity detection of the most challenging - rare, low-abundance and/or hidden antigens using fast 10-15-min. incubation protocols - also with weak, low affinity primary antibodies. With many regular, well accessible / abundant antigens and/or within longer incubation protocols AmpliStain™ reagents can be diluted to the smaller working strength concentrations that will still ensure highly sensitive detection. For that reason, along with AmpliStain™ reagents, we offer Universal IHC Diluent/Blocker/Stabilizer (#UDBS, a separately available item) as a complementary support product that can be used for diluting of all StrongZyme™ conjugates and, if wanted, primary antibodies. UDBS description Useful links and relevant publications: Signal amplification in immunohistochemistry: Loose-jointed deformable heteropolymeric HRP conjugates vs. linear polymer backbone HRP conjugates by Igor Buchwalow et al. in Acta Histochemica Volume 115, Issue 6, July 2013, Pages 587–594 http://www.sciencedirect.com/science/article/pii/S0065128112001833 http://dx.doi.org/10.1016/j.acthis.2012.12.008 |
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StrongZyme™ and Ready to Use RESERVE™ are Trademarks of SDT.