Binding/Coating Buffer 10X Concentrate #BBC

1 Volume #BBC mixed with 9 Volumes of pure deionized water gives
10 Volumes of 50mM Na-Carbonate/Bicarbonate Buffer pH 9.4
containing 0,03% 5-Bromo-5-nitro-1,3-dioxane which is an optimal universal Coating
Buffer for antibodies and vast majority of protein antigens in ELISA and related tests
that rely on physical adsorption of biomolecules onto hydrophobic and/or weakly
negative charged plastic surfaces.

#BBC is stable over two years at ambient room temperature. Do not store this product at
+4°C or otherwise at temperatures below 18°C. Longer exposition below 18°C may lead
to precipitation of
5-Bromo-5-nitro-1,3-dioxane into insoluble crystals.

Prepared from #BBC Coating Buffer will be stable two years at ambient room
temperature provided the water used in preparation has been 0.1/0.2µ-filtered (which is
a natural assumption as 0.1/0.2µ-filtration modules are integrated with all modern
water purification systems) and risk of contamination during preparation was minimized.

Binding/Coating Buffer 10X Concentrate    
                                                                                   #BBC
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Phosphate Buffered Saline (PBS) 10X Concentrate #PBSC

1 Volume #PBSC mixed with 9 Volumes of pure deionized water gives
10 Volumes of 150mM Phosphate Buffered Saline (120mM NaCl,
30mM Na/K-Phosphates) pH 7.3 containing 0,05%
5-Bromo-5-nitro-1,3-dioxane (PBS/BND). PBS is the most common buffer base in a
variety of liquid IVD reagents (see Standard Recommended Combinations at the end
of this section).

#PBSC is stable over two years at ambient room temperature. Do not store this product
at +4°C or otherwise at temperatures below 18°C. Longer exposition below 18°C may
result in falling out
5-Bromo-5-nitro-1,3-dioxane and phosphate crystals.

Prepared from this product PBS will be stable two years at ambient room temperature
provided the water used in preparation was 0.1/0.2µ-filtered (which is a due
assumption as 0.1/0.2µ-filtration modules are supplied with all modern water
purification systems) and risk of contamination during preparation was minimized.

Phosphate Buffered Saline (PBS) 10X Concentrate      #PBSC
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Wash Buffer 10X Concentrate #WBC

1 Volume #WBC mixed with 9 Volumes of pure deionized water yields 10 Volumes of
Wash Buffer comprising PBS/BND with 0,05%
Tween-20 (PBS-Tween) which is the most frequently used wash buffer in ELISA and
related applications.

#WBC is stable over two years at ambient room temperature. Do not store this product at
+4°C or otherwise at temperatures below 18°C. Longer exposition below 18°C may
provoke precipitation of
5-Bromo-5-nitro-1,3-dioxane and phosphates.

Prepared from this product Wash Buffer will be stable two years at ambient room
temperature provided the water used in preparation has been 0.1/0.2µ-filtered and risk
of contamination during preparation was minimized.

Wash Buffer 10X Concentrate                                      #WBC
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Substrate Buffer 10X Concentrate #SBC

1 Volume #SBS mixed with 9 Volumes of pure deionized water yields 10 Volumes of
Substrate Buffer which is a 100mM Na-Acetate/Citrate buffer pH 4,9 containing 0,02%
BND.

Recommended primarily as second wash/equilibrating buffer in 3D ImmunoFiltration
PolyHRP/p(HS)TMB format.

#SBC is stable over two years at ambient room temperature. Do not store this product at
+4°C or otherwise at temperatures below 18°C. Longer exposition below 18°C may
result in precipitation of
5-Bromo-5-nitro-1,3-dioxane.

Prepared from this product Wash Buffer will be stable two years at ambient room
temperature provided the water used in preparation was 0.1/0.2µ-filtered and risk of
contamination during preparation was minimized.

Substrate Buffer 10X Concentrate                                 #SBC
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Standard recommended combinations:

1) Blocking Reagent 1% BSA in PBS/BND:

1 Volume BSA1/2 + 0,9 (~1) Volumes #PBSC + 8,1 (~8) Volumes pure
deionized 0.1/0.2mm-filtered water

2) First after-conjugate Wash Reagent in 3D-ImmunoFiltration 0,2% BSA:
1 Volume BSA1/2 + 4,9 (~5) Volumes #PBSC + 44,1 (~45) Volumes
pure deionized 0.1/0.2mm-filtered water

3) Serum/Plasma Sample Diluents:

- for samples diluted 1/10 and higher:

1 Volume UB + 19 Volumes UCDB

- for samples diluted lower than 1/10:

1 Volume UB + 9 Volumes UCDB